Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | stimulates | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1S,3S)-3-acetyl-3,5,12-trihydroxy-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 3-amino-2,3,6-trideoxy-alpha-L-lyxo-hexopyranoside | - |
Saccharolobus shibatae | |
4'-(9-acridinylamino)methansulfon-m-anisidide | - |
Saccharolobus shibatae | |
4'-(9-acridinylamino)methansulfon-o-anisidide | - |
Saccharolobus shibatae | |
Amsarcines | - |
Saccharolobus shibatae | |
coumermycin | no inhibition | Saccharolobus shibatae | |
doxorubicin | - |
Saccharolobus shibatae | |
ellipticines | - |
Saccharolobus shibatae | |
NaCl | above 100 mM | Saccharolobus shibatae | |
novobiocin | no inhibition | Saccharolobus shibatae | |
quinolones | e.g. CP-115,953 | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharolobus shibatae | |
Mg2+ | Mg2+ is effective in the concentration range 1-40 mM, optimal concentration is around 30 mM | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000 + 2 * 60000, SDS-PAGE | Saccharolobus shibatae |
60000 | - |
2 * 47000 + 2 * 60000, SDS-PAGE | Saccharolobus shibatae |
230000 | - |
gel filtration, sucrose density gradient centrifugation | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus shibatae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharolobus shibatae |
Storage Stability | Organism |
---|---|
4°C, 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT, 0.0005% Triton X-100 | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | changes the linking number of a closed circular molecule by an increment of two | Saccharolobus shibatae | ? | - |
? | |
network of DNA rings + ATP + H2O | decatenation | Saccharolobus shibatae | monomeric DNA circles + ADP + phosphate | - |
? | |
network of DNA rings + ATP + H2O | enzyme is more efficient in decatenation than relaxation | Saccharolobus shibatae | monomeric DNA circles + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | catenation | Saccharolobus shibatae | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation | Saccharolobus shibatae | relaxed DNA + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | ATP-dependent relaxation of negative and positive supercoils | Saccharolobus shibatae | relaxed DNA + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 47000 + 2 * 60000, SDS-PAGE | Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | - |
Saccharolobus shibatae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: no activity, 70-80°C: maximal activity | Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
pH 7, enzyme concentration 90 ng/ml, less than 15% loss of activity after 40 min | Saccharolobus shibatae |
80 | - |
pH 7, enzyme concentration 90 ng/ml, half-life: about 35 min. At an enzyme concentration of 900 ng/ml less than 20% loss of activity after 40 min | Saccharolobus shibatae |
95 | - |
pH 7, enzyme concentration 90 ng/ml, more than 90% inactivation after 5 min | Saccharolobus shibatae |