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Literature summary for 5.6.2.2 extracted from

  • Halligan, B.D.; Edwards, K.A.; Liu, L.F.
    Purification and characterization of a type II DNA topoisomerase from bovine calf thymus (1985), J. Biol. Chem., 260, 2475-2482.
    View publication on PubMed
Search for more literature for 5.6.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 ATP ATPase reaction Bos taurus
0.4
-
 ATP in presence of supercoiled plasmid DNA Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 1 * 125000 + 1 * 140000, SDS-PAGE Bos taurus
140000
-
 1 * 125000 + 1 * 140000, SDS-PAGE Bos taurus
277000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
large scale Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information unknotts P4 DNA in an ATP-dependent manner Bos taurus ?
-
 ?
additional information DNA stimulated ATPase activity Bos taurus ?
-
 ?
network of DNA rings + ATP + H2O decatenation Bos taurus monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Bos taurus catenated DNA networks + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O relaxation Bos taurus relaxed DNA + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 125000 + 1 * 140000, SDS-PAGE Bos taurus