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Literature summary for 5.6.2.1 extracted from
- Insights from the structure of Mycobacterium tuberculosis topoisomerase I with a novel protein fold (2016), J. Mol. Biol., 428, 182-193 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli T7 Express Crystal cells | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris-HCl pH 8.5, and 25% (w/v) PEG3350 | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose 6 column chromatography and single-stranded DNA cellulose column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled pBAD/Thio plasmid DNA | - |
Mycobacterium tuberculosis | relaxced pBAD/Thio plasmid DNA | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA topoisomerase I | - |
Mycobacterium tuberculosis |
| Top1 | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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