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Literature summary for 5.6.2.1 extracted from

  • Viard, T.; Lamour, V.; Duguet, M.; Bouthier de la Tour, C.
    Hyperthermophilic topoisomerase I from Thermotoga maritima A very efficient enzyme that functions independently of zinc binding (2001), J. Biol. Chem., 276, 46495-46503 .
    View publication on PubMed
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
C559A/C561A no detectable zinc is bound to the mutant enzyme and its fluorescence properties are modified, suggesting a change of conformation in the protein lacking zinc. GThe mutant enzyme exhibits an activity similar to that of the wild-type enzyme with the same optimal temperature for activity. Thermostability is not affected by the mutation. Decatenase activity of wild type and C559A/C561A mutant enzymes shows no significant difference. Cleavage positions obtained with the C559A/C561A mutant is similar to those observed with the wild type enzyme. Preference for cytosine at position -4 is retained, and cleavage intensities are comparable Thermotoga maritima
Y288F totally inactive protein Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
additional information zinc binding is not required for DNA relaxation activity Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima P46799
-
-
Thermotoga maritima DSM 3109 P46799
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and mutant enzymes Y288F and C559A/C561A Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA the enzyme exhibits a very high DNA relaxing activity. Strong preference for a cytosine at position -4 of the cleavage Thermotoga maritima ?
-
 ?
DNA the enzyme exhibits a very high DNA relaxing activity. Strong preference for a cytosine at position -4 of the cleavage Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
Topoisomerase I
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Thermotoga maritima
70 75
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 both wild type and C559A/C561A mutant enzymes remain fully active after 30 min Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermotoga maritima