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Literature summary for 5.6.2.1 extracted from

  • Valenti, A.; de Felice, M.; Perugino, G.; Bizard, A.; Nadal, M.; Rossi, M.; Ciaramella, M.
    Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization (2012), J. Biol. Chem., 287, 30282-30295.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the lack of stimulation by ssDNA binding protein SSB suggests that ssDNA regions required for SsTop3 binding are stably exposed in the negatively supercoiled substrate at the high reaction temperature, even in the absence of ssDNA binding protein SSB Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled DNA ATP-independent relaxation of negatively supercoiled DNA. The lack of stimulation by ssDNA binding protein SSB suggests that ssDNA regions required for SsTop3 binding are stably exposed in the negatively supercoiled substrate at the high reaction temperature, even in the absence of ssDNA binding protein SSB Saccharolobus solfataricus ?
-
 ?

Synonyms

Synonyms Comment Organism
SsTop3
-
 Saccharolobus solfataricus
TopA
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Saccharolobus solfataricus