Inhibitors | Comment | Organism | Structure |
---|---|---|---|
single-stranded M13 DNA | inhibits relation of negatively supercoiled DNA at 60-80°C | Desulfurococcus amylolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely requires Mg2+ for activity | Desulfurococcus amylolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
gel filtration | Desulfurococcus amylolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfurococcus amylolyticus | - |
- |
- |
Desulfurococcus amylolyticus DSM 3822 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Desulfurococcus amylolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | specific for single-stranded DNA, the enzyme drives extensive unwinding of closed circular DNA at high temperature. At 60-80°C it relaxes negatively but not positively supercoiled DNA. At 95°C, the enzyme unwinds both positively and negatively supercoiled substrates and produces extensively unwound form I* and I** DNA | Desulfurococcus amylolyticus | ? | - |
? | |
DNA | specific for single-stranded DNA, the enzyme drives extensive unwinding of closed circular DNA at high temperature. At 60-80°C it relaxes negatively but not positively supercoiled DNA. At 95°C, the enzyme unwinds both positively and negatively supercoiled substrates and produces extensively unwound form I* and I** DNA | Desulfurococcus amylolyticus DSM 3822 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 108000, electrophoresis under denaturing conditions | Desulfurococcus amylolyticus |
Synonyms | Comment | Organism |
---|---|---|
ATP-independent type I topoisomerase | - |
Desulfurococcus amylolyticus |
Dam DNA topoisomerase III | - |
Desulfurococcus amylolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Desulfurococcus amylolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Desulfurococcus amylolyticus |