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Literature summary for 5.6.2.1 extracted from

  • Slesarev, A.I.; Zaitzev, D.A.; Kopylov, V.M.; Stetter, K.O.; Kozyavkin, S.A.
    DNA topoisomerase III from extremely thermophilic archaebacteria. ATP-independent type I topoisomerase from Desulfurococcus amylolyticus drives extensive unwinding of closed circular DNA at high temperature (1991), J. Biol. Chem., 266, 12321-12328.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
single-stranded M13 DNA inhibits relation of negatively supercoiled DNA at 60-80°C Desulfurococcus amylolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely requires Mg2+ for activity Desulfurococcus amylolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
gel filtration Desulfurococcus amylolyticus

Organism

Organism UniProt Comment Textmining
Desulfurococcus amylolyticus
-
-
-
Desulfurococcus amylolyticus DSM 3822
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Desulfurococcus amylolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA specific for single-stranded DNA, the enzyme drives extensive unwinding of closed circular DNA at high temperature. At 60-80°C it relaxes negatively but not positively supercoiled DNA. At 95°C, the enzyme unwinds both positively and negatively supercoiled substrates and produces extensively unwound form I* and I** DNA Desulfurococcus amylolyticus ?
-
?
DNA specific for single-stranded DNA, the enzyme drives extensive unwinding of closed circular DNA at high temperature. At 60-80°C it relaxes negatively but not positively supercoiled DNA. At 95°C, the enzyme unwinds both positively and negatively supercoiled substrates and produces extensively unwound form I* and I** DNA Desulfurococcus amylolyticus DSM 3822 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 108000, electrophoresis under denaturing conditions Desulfurococcus amylolyticus

Synonyms

Synonyms Comment Organism
ATP-independent type I topoisomerase
-
Desulfurococcus amylolyticus
Dam DNA topoisomerase III
-
Desulfurococcus amylolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Desulfurococcus amylolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Desulfurococcus amylolyticus