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Literature summary for 5.6.2.1 extracted from
- Topoisomerase I (TopA) is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation (2013), J. Bacteriol., 195, 4445-4455.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene topA, expression fo His-tagged enzyme under the control of a modified pET28 Plac promoter in Escherichia coli strain BL21 | Streptomyces coelicolor |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | TopA depletion in strains PS01 (wild-type background) and PS03 (-parB background). TopA depletion in the -parB background (PS03) leads to a decrease in chromosome segregation abnormalities with 8.9% anucleate prespores compared to that in the parB deletion strain J3305, with 19.5% anucleate prespores | Streptomyces coelicolor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Streptomyces coelicolor | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
topoisomerase I, TopA, is the only topoisomerase I homologue in the organism | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Streptomyces coelicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypha | aerial | Streptomyces coelicolor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate is pUC19 plasmid DNA, and for supercoiling assay substrate pWHM3 plasmid DNA | Streptomyces coelicolor | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TopA | - |
Streptomyces coelicolor |
| Topoisomerase I | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Streptomyces coelicolor |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the enzyme depletion inhibits growth, blocks sporulation, and affects septation and chromosome condensation. But sporulation in the TopA-depleted strain can be partially restored by deletion of parB. Depletion of the TopA protein affects the secondary metabolism of Streptomyces coelicolor, leading to overproduction of actinorhodin | Streptomyces coelicolor |
| physiological function | topoisomerase I is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation, overview | Streptomyces coelicolor |
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