Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain RLM1180/pTI-1 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
0.00000752 | - |
negatively supercoiled plasmid pXXZ06 DNA | pH 7.9, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by polymin P and ammonium sulfate fractionations, cation- and anion-exchange chromatography, purified DNA topoisomerase I is free of DNase contamination, method development, overview | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31700 | - |
purified enzyme, pH 7.9, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
negatively supercoiled plasmid pXXZ06 DNA | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase I | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
negatively supercoiled plasmid pXXZ06 DNA | pH 7.9, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Escherichia coli |