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Literature summary for 5.6.2.1 extracted from

  • Banerjee, B.; Sen, N.; Majumder, H.K.
    Identification of a functional type IA topoisomerase, LdTopIIIbeta, from kinetoplastid parasite Leishmania donovani (2011), Enzyme Res., 2011, 230542.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
two genes encoded in ORFs LmjF28.1780 and LmjF36.3200, DNA and amino acid sequence determination and analysis, sequence comparisons. TopIIIbeta subcloning in Escherichia coli DH5alpha, expression in Escherichia coli strain BL21 and in Saccharomyces cerevisiae strain W5909-3B. Expression of C-terminal-GFP-tagged full length LdTopIII? protein in Leishmania donovani promastigotes. LdTopIIIbeta suppresses the yeast top3DELTA slow-growth phenotype Leishmania donovani

Protein Variants

Protein Variants Comment Organism
Y327F site-directed mutagenesis, the active site mutant construct cannot suppress the slow-growth of top3 mutant Saccharomyces cerevisiae Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast LdTopIIIbeta protein localized both inside the nucleus and kinetoplast of the parasites Leishmania donovani 20023
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nucleus LdTopIIIbeta protein localized both inside the nucleus and kinetoplast of the parasites Leishmania donovani 5634
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ TopIIIbeta shows strict dependence on magnesium Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leishmania donovani TopIIIbeta possesses a weak, ATP-independent relaxation activity towards negatively supercoiled DNA only ?
-
 ?
additional information Leishmania donovani AG 83 TopIIIbeta possesses a weak, ATP-independent relaxation activity towards negatively supercoiled DNA only ?
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania donovani D0EG00 contains two topoisomerase III genes
-
Leishmania donovani AG 83 D0EG00 contains two topoisomerase III genes
-

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TopIIIbeta possesses a weak, ATP-independent relaxation activity towards negatively supercoiled DNA only Leishmania donovani ?
-
 ?
additional information the active site tyrosine is located at the 327 position within a highly conserved GYISYPRTES sequence Leishmania donovani ?
-
 ?
additional information TopIIIbeta possesses a weak, ATP-independent relaxation activity towards negatively supercoiled DNA only Leishmania donovani AG 83 ?
-
 ?
additional information the active site tyrosine is located at the 327 position within a highly conserved GYISYPRTES sequence Leishmania donovani AG 83 ?
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional ribbon structure of LdTopIIIbeta generated based on the crystal structure of Escherichia coli topo isomerase III, overview Leishmania donovani

Synonyms

Synonyms Comment Organism
LdTopIIIbeta
-
 Leishmania donovani
type IA topoisomerase
-
 Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for ATP Leishmania donovani

General Information

General Information Comment Organism
evolution genes encoding topoisomerase III enzymes are highly conserved in evolution from bacteria to human Leishmania donovani
malfunction the phenotypic consequences of loss of topoisomerase III function are generally quite severe Leishmania donovani
additional information LdTopIIIbeta suppresses the yeast top3DELTA slow-growth phenotype. The C-terminal domain of LdTopIIIbeta is essential for in vivo complementation Leishmania donovani