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Literature summary for 5.6.2.1 extracted from

  • Fiorani, P.; Tesauro, C.; Mancini, G.; Chillemi, G.; D'Annessa, I.; Graziani, G.; Tentori, L.; Muzi, A.; Desideri, A.
    Evidence of the crucial role of the linker domain on the catalytic activity of human topoisomerase I by experimental and simulative characterization of the Lys681Ala mutant (2009), Nucleic Acids Res., 37, 6849-6858.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged K681A mutant enzyme in Saccharomyces cerevisiae Homo sapiens

Protein Variants

Protein Variants Comment Organism
K681A site-directed mutagenesis, the mutation of Lys681, located on the tip of the linker domain, dramatically reduces the religation rate, the equilibrium of the mutant is shifted toward cleavage when compared to the wild-type enzyme. Mutation of residue 681 from lysine to alanine does not cause alterations in the linker domain secondary structure, and the main chain conformation of the mutated residue does not significantly change in relation with the neighbor residues, when compared to the wild-type protein Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens DNA topoisomerase IB catalyzes the relaxation of supercoiled DNA through the transient cleavage of one strand of a DNA duplex, molecular dynamics simulation of wild-type and mutant K681A enzymes, the N-terminal and C-terminal regions of the core domain show a slightly larger and a slightly smaller displacements, respectively, in the wild type than in the mutant enzyme, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DNA topoisomerase IB catalyzes the relaxation of supercoiled DNA through the transient cleavage of one strand of a DNA duplex, molecular dynamics simulation of wild-type and mutant K681A enzymes, the N-terminal and C-terminal regions of the core domain show a slightly larger and a slightly smaller displacements, respectively, in the wild type than in the mutant enzyme, overview Homo sapiens ?
-
 ?
additional information cleavage of oligonucleotide substrates 5'-GAAAAAAGACTTAG-3' and 5'-TAAAAATTTTTCTAAGTCTTTTTTC-3'. Crucial role for the linker domain and occurrence of a long range communication between the linker and the active site region, overview Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase IB
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 cleavage assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 cleavage assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme is of fundamental importance to processes such as replication, recombination and transcription Homo sapiens