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Literature summary for 5.6.2.1 extracted from
- Mechanistic studies of the modulation of cleavage activity of topoisomerase I by DNA adducts of mono- and bi-functional PtII complexes (2009), Nucleic Acids Res., 37, 5432-5442.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| camptothecin | - |
Homo sapiens |  |
| cisplatin | presence of DNA adducts of bifunctional antitumor cisplatin or monodentate [PtCl(dien)]Cl in the substrate DNA inhibits eukaryotic top1 action, the adducts of cisplatin being more effective | Homo sapiens | |
| monodentate [PtCl(diethylenetriamine)]Cl | presence of DNA adducts of bifunctional antitumor cisplatin or monodentate [PtCl(dien)]Cl in the substrate DNA inhibits eukaryotic top1 action, the adducts of cisplatin being more effective. Inhibition is markedly enhanced by camptothecin. Sequence preference of Pt-DNA adducts in the 161-bp substrate DNA fragment. Inhibition mechanism and interaction analysis, overview | Homo sapiens | |
| additional information | a multiple metallation of the short nucleotide sequences on the scissile strand, immediately downstream of the cleavage site impedes the cleavage by top1 | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate is supercoiled plasmid pSP73KB DNA, a multiple metallation of the short nucleotide sequences on the scissile strand, immediately downstream of the cleavage site impedes the cleavage by top1. Top1-mediated cleavage of a 30-bp oligonucleotide duplex | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Topoisomerase I | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | eukaryotic top1 is affected by the modification of DNA by antitumor cisplatin | Homo sapiens |
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