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Literature summary for 5.6.2.1 extracted from
- Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria (2006), J. Virol., 80, 314-321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)-RIL | Acanthamoeba polyphaga Mimivirus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Acanthamoeba polyphaga Mimivirus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H285A | display a low level of supercoil relaxation. Slow the rate of cleavage by a factor of 60 compared to the wild-type | Acanthamoeba polyphaga Mimivirus |
| additional information | conclude that the active sites of MimiTopIB and vaccinia TopIB are composed of similar functional groups | Acanthamoeba polyphaga Mimivirus |
| R135A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
| R241A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
| Y294A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE | Acanthamoeba polyphaga Mimivirus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba polyphaga Mimivirus | - |
a parasite of Acanthamoeba polyphaga | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from a soluble bacterial lysate by Ni2+-agarose chromatography | Acanthamoeba polyphaga Mimivirus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | The sedimentation results are consistent with MimiTopIB being a monomer in solution, although the prospect that MimiTopIB is an asymmetrically shaped homodimer cannot excluded | Acanthamoeba polyphaga Mimivirus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA topoisomerase IB | - |
Acanthamoeba polyphaga Mimivirus |
| MimiTopIB | - |
Acanthamoeba polyphaga Mimivirus |
| TopIB | - |
Acanthamoeba polyphaga Mimivirus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | relaxes DNA over a broad pH range | Acanthamoeba polyphaga Mimivirus |
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