Literature summary for 5.6.2.1 extracted from

Hede, M.S.; Petersen, R.L.; Froehlich, R.F.; Krueger, D.; Andersen, F.F.; Andersen, A.H.; Knudsen, B.R.
Resolution of Holliday junction substrates by human topoisomerase I (2007), J. Mol. Biol., 365, 1076-1092.

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Protein Variants

Protein Variants	Comment	Organism
hTopoI (deltaNL)	hTopoI deletion mutant. Considering the domain organization, hTopoI distinguishes itself from both Vaccinia virus TopoI and the tyrosine recombinases, by having rather extensive N-terminal and linker domains. Investigation of the resolution activity of hTopoI deleted in either one or both of these domains. Two hTopoI variants, hTopoI(deltaN) lacking aa 1-202, and hTopoI(deltaNL) lacking aa 1-202 and 660-688. Deleting the N-terminal and linker domains of hTopoI in order to mimic the domain organization of Vaccinia virus TopoI and the tyrosine recombinases inhibited resolution of the Holliday junction substrate. hTopoI(deltaNL) forms dimers	Homo sapiens
hTopoI(deltaN)	hTopoI deletion mutant. Considering the domain organization, hTopoI distinguishes itself from both Vaccinia virus TopoI and the tyrosine recombinases, by having rather extensive N-terminal and linker domains. Investigation of the resolution activity of hTopoI deleted in either one or both of these domains. Two hTopoI variants, hTopoI(deltaN) lacking aa 1-202, and hTopoI(deltaNL) lacking aa 1-202 and 660-688. Deleting the N-terminal and linker domains of hTopoI in order to mimic the domain organization of Vaccinia virus TopoI and the tyrosine recombinases inhibited resolution of the Holliday junction substrate. hTopoI(deltaNL) forms dimers	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Holliday Junction Substrate H1-4	close relationship between tyrosine recombinases and type IB topoisomerases, investigation of ability of human topoisomerase I to resolve the typical intermediate of recombinase catalysis, the Holliday junction, results consolidate the relationship between type IB topoisomerases and tyrosine recombinases	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
hTopoI	-	Homo sapiens
Topoisomerase I	-	Homo sapiens

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Release 2023.1 (January 2023)