Literature summary for 5.6.2.1 extracted from

Diaz Gonzalez, R.; Perez Pertejo, Y.; Redondo, C.M.; Pommier, Y.; Balana-Fouce, R.; Reguera, R.M.
Structural insights on the small subunit of DNA topoisomerase I from the unicellular parasite Leishmania donovani (2007), Biochimie, 89, 1517-1527.

Search for more literature for 5.6.2.1

Application

Application	Comment	Organism
drug development	DNA topoisomerase I is an important cellular target in drug discovery	Leishmania donovani

Cloned(Commentary)

Cloned (Comment)	Organism
Heterologous co-expression of LdTop1L and LdTop1S in a topoisomerase I deficient Saccharomyces cerevisiae strain (EKY3, MBY3), reconstitutes a fully functional enzyme which can be used for structural studies. Purified fragments of N-terminal truncation are used to transform XL-1Blue Escherichia coli	Leishmania donovani

Protein Variants

Protein Variants	Comment	Organism
delta 175-262	N-terminal truncation performed on LdTop1S subunits	Leishmania donovani
delta 180-262	N-terminal truncation performed on LdTop1S subunits	Leishmania donovani
delta 182-262	N-terminal truncation performed on LdTop1S subunits	Leishmania donovani
delta 200-262	N-terminal truncation performed on LdTop1S subunits	Leishmania donovani
delta 210-262	N-terminal truncation performed on LdTop1S subunits	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
camptothecin	Wild type and mutant sensitivity to camptothecin is determined in DNA cleavage assays. In vivo sensitivity to camptothecin also measured	Leishmania donovani
additional information	trapping of the covalent complex between LdTop1 and DNA by camptothecin resides in a pentapeptide placed upwards Arg-180, increases the sensitivity to the poison	Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	Two different subunits, small protein of 28000 (LdTop1S)	Leishmania donovani
73000	-	Two different subunits, large protein of 73000 (LdTop1L)	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
SDS-PAGE	Leishmania donovani

Source Tissue

Source Tissue	Comment	Organism	Textmining

Subunits

Subunits	Comment	Organism
heterodimer	unique heterodimeric DNA topoisomerase IB, consists of a large subunit (LdTop1L), which contains the non-conserved N-terminal end and a phylogenetically conserved core domain, and of a small subunit (LdTop1S) which harbours the C-terminal region with a characteristic tyrosine residue in the active site	Leishmania donovani
More	important role by part of the N-terminal extension of the small subunit of DNA topoisomerase I, contribute with a main portion of the linker domain of this enzyme	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
DNA topoisomerase I	-	Leishmania donovani
LdTop1	-	Leishmania donovani

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Release 2023.1 (January 2023)