Literature summary for 5.6.2.1 extracted from

Interthal, H.; Quigley, P.M.; Hol, W.G.; Champoux, J.J.
The role of lysine 532 in the catalytic mechanism of human topoisomerase I (2004), J. Biol. Chem., 279, 2984-2992.

Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes in Saccharomyces cerevisiae	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
topo70 K532R/Y723F protein in complex with a 22-mer duplex DNA oligonucleotide, vapour diffusion at room. Cell dimensions: a = 56.9 A, b = 118.6 A, c = 71.7 A, beta = 98.3°	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K532A	cleavage and religation activity of the mutant enzyme are reduced, religation is reduced to a greater extent than cleavage	Homo sapiens
K532A	expression of the mutant enzyme in Saccharomyces cerevisiae is cytotoxic, this mutant enzyme mimics the effects of the anticancer drug camptothecin	Homo sapiens
K532R	cleavage and religation activity of the mutant enzyme are reduced, cleavage is reduced to a greater extent than religation	Homo sapiens
Y723F	mutation renders the topoisomerase completely inactive	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P11387	-	-

Purification (Commentary)

Purification (Comment)	Organism
mutant enzymes	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Topoisomerase I	-	Homo sapiens

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Release 2023.1 (January 2023)