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Literature summary for 5.6.2.1 extracted from

  • Suzuki, M.; Takagi, E.; Kojima, K.; Izuta, S.; Yoshida, S.
    Rapid purification and structural study of DNA topoisomerase I from human Burkitt lymphoma Raji cells (1989), J. Biochem., 106, 742-744.
    View publication on PubMed
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
camptothecins
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no requirement for Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 glycerol density gradient centrifugation, monomeric form Homo sapiens
200000
-
 gel filtration, dimeric form Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Burkitt lymphoma cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Homo sapiens relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 100000, SDS-PAGE. DNA topoisomerase I exists largely in a monomeric form, but also has a minor population of the dimeric form Homo sapiens
monomer 1 * 100000, SDS-PAGE. DNA topoisomerase I exists largely in a monomeric form, but also has a minor population of the dimeric form Homo sapiens