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Literature summary for 5.6.2.1 extracted from
- Characterization of vaccinia virus DNA topoisomerase I expressed in Escherichia coli (1988), J. Biol. Chem., 263, 16401-16407.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermidine | enhances relaxation activity | Vaccinia virus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli under the control of a bacteriophage T7 promoter | Vaccinia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| camptothecins | no inhibition | Vaccinia virus | |
| Cu2+ | - |
Vaccinia virus | |
| Zn2+ | - |
Vaccinia virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Vaccinia virus | |
| Co2+ | stimulates | Vaccinia virus | |
| Mg2+ | stimulates | Vaccinia virus | |
| Mg2+ | optimal concentration is about 5 mM | Vaccinia virus | |
| Mn2+ | stimulates | Vaccinia virus | |
| Na+ | monovalent cation required, in presence of EDTA optimal activity between 100 and 250 mM NaCl | Vaccinia virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | 33000 | glycerol density gradient centrifugation, gel filtration | Vaccinia virus |
| 33000 | - |
1 * 33000, SDS-PAGE | Vaccinia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled DNA | relaxation | Vaccinia virus | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | positive and negative supercoiled DNA | Vaccinia virus | relaxed closed circular DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, SDS-PAGE | Vaccinia virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 5 min, stable | Vaccinia virus |
| 65 | - |
partial inactivation | Vaccinia virus |
| 75 | - |
5 min, inactivated | Vaccinia virus |
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