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Literature summary for 5.6.2.1 extracted from

  • Ichishima, E.; Yoshida, T.; Habuka, N.; Takeuchi, M.; Arai, T.
    Mode of action of DNA topoisomerase I from Alcaligenes sp. (1985), Biochim. Biophys. Acta, 826, 81-86.
No PubMed abstract available
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
Ethidium bromide
-
 Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Mg2+ or Ca2+ required Alcaligenes sp.
Mg2+ optimal concentration is about 5 mM Alcaligenes sp.
Mg2+ Mg2+ or Ca2+ required Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA removes superhelical turns from a negatively twisted DNA, no effect on positive superhelical turns Alcaligenes sp. relaxed closed circular DNA
-
 ?
supercoiled DNA relaxation Alcaligenes sp. relaxed closed circular DNA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 7.5, 10 min, stable Alcaligenes sp.
60
-
 pH 7.5, 10 min, complete inactivation Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10
-
 Alcaligenes sp.