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Literature summary for 5.6.2.1 extracted from

  • Liu, L.F.
    HeLa topoisomerase I (1983), Methods Enzymol., 100, 133-137.
    View publication on PubMed
Search for more literature for 5.6.2.1

Activating Compound

Activating Compound Comment Organism Structure
histone H1 stimulates Homo sapiens
Nonhistone HMG proteins stimulates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ active also in absence of Mg2+, but with 5fold to 50fold reduced activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme breaks down single-stranded DNA to smaller fragments. Each cleavage generates a free 5'-OH end and an enzyme-linked 3'-phosphoryl end Homo sapiens ?
-
 ?
Nicked circular DNA catenation reaction Homo sapiens Large catenated DNA networks
-
 ?
supercoiled DNA rather weak sequence specificity Homo sapiens relaxed closed circular DNA
-
 ?
supercoiled DNA relaxation Homo sapiens relaxed closed circular DNA
-
 ?
supercoiled DNA positive and negative supercoiled DNA Homo sapiens relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Homo sapiens