Literature summary for 5.6.1.9 extracted from

Kim, J.; Darley, D.; Buckel, W.
2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile (2005), FEBS J., 272, 550-561 .

Search for more literature for 5.6.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	Q5U925	-	-

Oxidation Stability

Oxidation Stability	Organism
protein is extremely sensitive in the presence of oxygen	Clostridioides difficile

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ATP + a reduced flavodoxin + inactive 2-hydroxyisocaproyl-CoA dehydratase + 2 H2O	-	Clostridioides difficile	2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyisocaproyl-CoA dehydratase	-	?

Synonyms

Synonyms	Comment	Organism
2-hydroxyisocaproyl-CoA dehydratase activator	-	Clostridioides difficile
hadI	-	Clostridioides difficile

Cofactor

Cofactor	Comment	Organism	Structure
4Fe-4S-center	protein contains 4 mol nonheme iron and 2 mol acid-labile sulfur per mol	Clostridioides difficile

General Information

General Information	Comment	Organism
physiological function	the reduced activator HadI transfers one electron to the dehydratase concomitant with hydrolysis of ATP. The thus activated dehydratase catalyzes about 10000 dehydration turnovers until the enzyme becomes inactive. Adding activator, ATP, MgCl2, dithionite and dithioerythritol reactivates the enzyme	Clostridioides difficile

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Release 2023.1 (January 2023)