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Literature summary for 5.6.1.9 extracted from

  • Mueller, U.; Buckel, W.
    Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans (1995), Eur. J. Biochem., 230, 698-704 .
    View publication on PubMed
Search for more literature for 5.6.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acidaminococcus fermentans

General Stability

General Stability Organism
glycerol, Fe2+, and several reducing agents in the purification buffers has no stabilizing effect Acidaminococcus fermentans
recombinant protein is very unstable, the use of proteinase inhibitors, Acidaminococcus fermentans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Acidaminococcus fermentans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron HgdC contains 4 mol non-heme iron and 3 mol inorganic sulfur per mol Acidaminococcus fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Acidaminococcus fermentans

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans P11568
-
-
Acidaminococcus fermentans DSM 20731 P11568
-
-

Oxidation Stability

Oxidation Stability Organism
under air, the activator has a half-life of seconds and even under strict anaerobic conditions it is very unstable Acidaminococcus fermentans

Purification (Commentary)

Purification (Comment) Organism
-
 Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2000
-
 pH not specified in the publication, temperature not specified in the publication Acidaminococcus fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O only catalytic amounts of activator HgdC are required for complete activation of the dehydratase Acidaminococcus fermentans 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O only catalytic amounts of activator HgdC are required for complete activation of the dehydratase Acidaminococcus fermentans DSM 20731 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?

Subunits

Subunits Comment Organism
dimer 2 27500, calculated from sequence Acidaminococcus fermentans

Synonyms

Synonyms Comment Organism
HDC
-
 Acidaminococcus fermentans