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Literature summary for 5.6.1.9 extracted from

  • Schweiger, G.; Dutscho, R.; Buckel, W.
    Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein (1987), Eur. J. Biochem., 169, 441-448 .
    View publication on PubMed
Search for more literature for 5.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibitory above 0.15 mM. 40% inhibition at 0.6 mM Acidaminococcus fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000 60000 gel filtration Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O Acidaminococcus fermentans
-
 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O Acidaminococcus fermentans DSM 20731
-
 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans P11568
-
-
Acidaminococcus fermentans DSM 20731 P11568
-
-

Oxidation Stability

Oxidation Stability Organism
the activator protein is irreversibly inactivated by exposure to air within less than 15 min Acidaminococcus fermentans

Purification (Commentary)

Purification (Comment) Organism
-
 Acidaminococcus fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O
-
 Acidaminococcus fermentans 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O
-
 Acidaminococcus fermentans DSM 20731 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
additional information the activation of the dehydratase requires only ATP Acidaminococcus fermentans ?
-
 ?
additional information the activation of the dehydratase requires only ATP Acidaminococcus fermentans DSM 20731 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 25000, SDS-PAGE Acidaminococcus fermentans