Cloned (Comment) | Organism |
---|---|
His-tagged motor domain construct comprising amino acids 1-761 | Dictyostelium discoideum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole | - |
Dictyostelium discoideum | |
2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole | - |
Dictyostelium discoideum | |
2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol | - |
Dictyostelium discoideum | |
2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol | - |
Dictyostelium discoideum | |
2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol | - |
Dictyostelium discoideum | |
2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol | - |
Dictyostelium discoideum | |
2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol | - |
Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | P08799 | His-tagged motor domain construct comprising amino acids 1-761 | - |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + myosin bound to actin filament at position n | - |
Dictyostelium discoideum | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
myosin 2 | - |
Dictyostelium discoideum |
myosin 2 motor domain | - |
Dictyostelium discoideum |
myosin ATPase | - |
Dictyostelium discoideum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0244 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol | |
0.0472 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol | |
0.0919 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole | |
0.1254 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol | |
0.1263 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol | |
0.1822 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol | |
0.2745 | - |
pH not specified in the publication, 25°C | Dictyostelium discoideum | 2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole |