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Literature summary for 5.6.1.8 extracted from

  • Preller, M.; Chinthalapudi, K.; Martin, R.; Knolker, H.J.; Manstein, D.J.
    Inhibition of myosin ATPase activity by halogenated pseudilins: a structure-activity study (2011), J. Med. Chem., 54, 3675-3685.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged motor domain construct comprising amino acids 1-761 Dictyostelium discoideum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole
-
Dictyostelium discoideum
2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole
-
Dictyostelium discoideum
2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
-
Dictyostelium discoideum
2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
-
Dictyostelium discoideum
2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
-
Dictyostelium discoideum
2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol
-
Dictyostelium discoideum
2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
-
Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P08799 His-tagged motor domain construct comprising amino acids 1-761
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + myosin bound to actin filament at position n
-
Dictyostelium discoideum ADP + phosphate + myosin bound to actin filament at position n+1
-
?

Synonyms

Synonyms Comment Organism
myosin 2
-
Dictyostelium discoideum
myosin 2 motor domain
-
Dictyostelium discoideum
myosin ATPase
-
Dictyostelium discoideum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0244
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
0.0472
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
0.0919
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole
0.1254
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
0.1263
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol
0.1822
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
0.2745
-
pH not specified in the publication, 25°C Dictyostelium discoideum 2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole