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Literature summary for 5.6.1.8 extracted from

  • Amitani, I.; Sakamoto, T.; Ando, T.
    Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor (2001), Biophys. J., 80, 379-397.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.8

Activating Compound

Activating Compound Comment Organism Structure
actin
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000027
-
 actin pH 7.6, 25°C, cosubstrate GTP Oryctolagus cuniculus
0.000008
-
 actin pH 7.6, 25°C, cosubstrate ITP Oryctolagus cuniculus
0.0000096
-
 actin pH 7.6, 25°C, presence of Sr2+ at 2 mM Oryctolagus cuniculus
0.000012
-
 actin pH 7.6, 25°C, cosubstrate TTP Oryctolagus cuniculus
0.000017
-
 actin pH 7.6, 25°C, presence of Ni2+ at 2 mM Oryctolagus cuniculus
0.000022
-
 actin pH 7.6, 25°C, presence of Mn2+ at 2 mM Oryctolagus cuniculus
0.000028
-
 actin pH 7.6, 25°C, cosubstrate CTP Oryctolagus cuniculus
0.000029
-
 actin pH 7.6, 25°C, cosubstrate ATP Oryctolagus cuniculus
0.000029
-
 actin pH 7.6, 25°C, presence of Mg2+ at 2 mM Oryctolagus cuniculus
0.00007
-
 actin pH 7.6, 25°C, cosubstrate UTP Oryctolagus cuniculus
0.0009
-
 ATP pH 7.6, 25°C Oryctolagus cuniculus
0.11
-
 CTP pH 7.6, 25°C Oryctolagus cuniculus
0.2
-
 UTP pH 7.6, 25°C Oryctolagus cuniculus
0.25
-
 TTP pH 7.6, 25°C Oryctolagus cuniculus
0.5
-
 GTP pH 7.6, 25°C Oryctolagus cuniculus
1
-
 ITP pH 7.6, 25°C Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Oryctolagus cuniculus
Mn2+ activates Oryctolagus cuniculus
Ni2+ activates Oryctolagus cuniculus
Sr2+ activates Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + myosin bound to actin filament at position n = ADP + phosphate + myosin bound to actin filament at position n+1 kinetics and mechanical behaviour Oryctolagus cuniculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + actin
-
 Oryctolagus cuniculus ADP + phosphate + actin
-
 ?
CTP + H2O + actin
-
 Oryctolagus cuniculus CDP + phosphate + actin
-
 ?
gTP + H2O + actin
-
 Oryctolagus cuniculus GDP + phosphate + actin
-
 ?
ITP + H2O + actin
-
 Oryctolagus cuniculus IDP + phosphate + actin
-
 ?
TTP + H2O + actin
-
 Oryctolagus cuniculus TDP + phosphate + actin
-
 ?
UTP + H2O + actin
-
 Oryctolagus cuniculus UDP + phosphate + actin
-
 ?