Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus-RIL cells | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
D93K | the mutation of the beta subunit blocks ATP hydrolysis. The mutant has 43% of wild type protein refolding activity | Thermoplasma acidophilum |
D94A | the mutation of the alpha subunit blocks ATP hydrolysis. The mutant has 32% of wild type protein refolding activity | Thermoplasma acidophilum |
D94K | the mutation of the alpha subunit blocks ATP hydrolysis. The mutant has 31% of wild type protein refolding activity | Thermoplasma acidophilum |
T157A | the mutation of the alpha subunit blocks ATP binding. The mutant has 30.5% of wild type protein refolding activity | Thermoplasma acidophilum |
T158A | the mutation of the beta subunit blocks ATP binding. The mutant has 62.6% of wild type protein refolding activity | Thermoplasma acidophilum |
T96V | the mutation of the beta subunit blocks ATP binding. The mutant has 44% of wild type protein refolding activity | Thermoplasma acidophilum |
T97V | the mutation of the alpha subunit blocks ATP binding. The mutant has no activity | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
ATP | wild type enzyme, with rhamnose dehydrogenase as protein substrate, at pH 7.5 and 55°C | Thermoplasma acidophilum | |
0.082 | - |
ATP | mutant enzyme D94A, with rhamnose dehydrogenase as protein substrate, at pH 7.5 and 55°C | Thermoplasma acidophilum | |
0.285 | - |
ATP | mutant enzyme D93K, with rhamnose dehydrogenase as protein substrate, at pH 7.5 and 55°C | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 50 mM used in assay conditions | Thermoplasma acidophilum | |
Mg2+ | 20 mM used in assay conditions | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Thermoplasma acidophilum | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | P48424 | subunit alpha | - |
Thermoplasma acidophilum | P48425 | subunit beta | - |
Purification (Comment) | Organism |
---|---|
Superose 6 column chromatography | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + folded aldohexose dehydrogenase | - |
Thermoplasma acidophilum | ADP + phosphate + unfolded aldohexose dehydrogenase | - |
? | |
ATP + H2O + folded rhamnose dehydrogenase | - |
Thermoplasma acidophilum | ADP + phosphate + unfolded rhamnose dehydrogenase | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexadecamer | - |
Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
chaperonin | - |
Thermoplasma acidophilum |
thermosome | - |
Thermoplasma acidophilum |