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Literature summary for 5.6.1.7 extracted from

  • Stuart, S.F.; Leatherbarrow, R.J.; Willison, K.R.
    A two-step mechanism for the folding of actin by the yeast cytosolic chaperonin (2011), J. Biol. Chem., 286, 178-184.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
molecular biology the development of a spectroscopic actin folding assay that allows actin folding by CCT to be monitored in real time through labeling with the environmentally sensitive dye acrylodan. This approach allows greater sensitivity in the measurement of actin folding kinetics than gel electrophoresis-based assays, and is used to observe the effects of ATP concentration and temperature on the rate of actin folding Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
G345D mutation in subunit CCT4, located in the external region of the apical domain, causes actin defects in the temperature-sensitive yeast strain anc2-1. Using the spectroscopic folding assay, the rate of release of AcrylAct1 by CCT4anc2 is found to be essentially the same as for wild-type CCT at 2 mM ATP but different at higher concentrations Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a novel spectroscopic assay through selectively labeling the C terminus of yeast actin with acrylodan and observe significant changes in the acrylodan fluorescence emission spectrum as actin is chemically unfolded and then refolded by the chaperonin. Variation in the polarity of the environment surrounding the fluorescent probe during the unfolding/folding processes allows to monitor actin as it folds on CCT Saccharomyces cerevisiae ?
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Subunits

Subunits Comment Organism
hexadecamer
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Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
CCT
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Saccharomyces cerevisiae
chaperonin containing TCP1
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Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae