Application | Comment | Organism |
---|---|---|
molecular biology | the development of a spectroscopic actin folding assay that allows actin folding by CCT to be monitored in real time through labeling with the environmentally sensitive dye acrylodan. This approach allows greater sensitivity in the measurement of actin folding kinetics than gel electrophoresis-based assays, and is used to observe the effects of ATP concentration and temperature on the rate of actin folding | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
G345D | mutation in subunit CCT4, located in the external region of the apical domain, causes actin defects in the temperature-sensitive yeast strain anc2-1. Using the spectroscopic folding assay, the rate of release of AcrylAct1 by CCT4anc2 is found to be essentially the same as for wild-type CCT at 2 mM ATP but different at higher concentrations | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a novel spectroscopic assay through selectively labeling the C terminus of yeast actin with acrylodan and observe significant changes in the acrylodan fluorescence emission spectrum as actin is chemically unfolded and then refolded by the chaperonin. Variation in the polarity of the environment surrounding the fluorescent probe during the unfolding/folding processes allows to monitor actin as it folds on CCT | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexadecamer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
CCT | - |
Saccharomyces cerevisiae |
chaperonin containing TCP1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |