Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | strongly competitively inhibits PfCPN ATPase activity. Addition of an equimolar amount of ADP results in a 48% inhibition of ATPase activity. When ADP concentration is four times that of ATP, the PfCPN ATPase activity decreases to 10% of that without ADP. Inhibition of PfCPN ATPase decreases its chaperonin activity in protecting lysozyme from heat-induced inactivation | Pyrococcus furiosus | |
AMP | when AMP concentration is 8 times that of ATP, AMP inhibits the ATPase activity to 48% | Pyrococcus furiosus | |
Na+ | ATPase activity is a little lower in the presence of 0.3 M Na+ plus 0.3 M K+, than in the presence of 0.3 M K+ alone | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
ATP | pH 7.5, 85°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | relatively low ATPase activity in presence of Co2+ or Mn2+ | Pyrococcus furiosus | |
K+ | strong ATPase activity in presence of K+, optimal concentration: 2 M | Pyrococcus furiosus | |
Mg2+ | ATPase activity depends on K+ and Mg2+, optimal concentration: 5-7.5 mM Mg2+ | Pyrococcus furiosus | |
Mn2+ | relatively low ATPase activity in presence of Co2+ or Mn2+ | Pyrococcus furiosus | |
additional information | in the presence of Cd2+, Ba2+, Ca2+ or Sr2+, little ATPase activity occurrs. Little activity is found in the presence of Na+ or Li+ | Pyrococcus furiosus | |
NH4+ | strong ATPase activity in presence of NH4+ | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | chaperonin activity in protecting lysozyme from heat-induced inactivation | Pyrococcus furiosus | ADP + phosphate + an unfolded polypeptide | - |
? | |
additional information | PfCPN has almost no ADPase activity | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PfCPN | - |
Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 6.5: about 30% of maximal activity, pH 7.0: about 90% of maximal activity, pH 9.0: about 60% of maximal activity | Pyrococcus furiosus |