Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | - |
Thermoplasma acidophilum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required for ATP hydrolysis, mediate nucleoside triphosphate-dependent refolding of malate dehydrogenase | Thermoplasma acidophilum | |
Mg2+ | highest ATP hydrolysis activity, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum | |
Mn2+ | divalent cation required for ATP hydrolysis, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation, increased substrate protein binding of Ta-cpn alpha | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | 60°C, assay at pH 7.0, 90 min, 0,12 mM released phosphate | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | folding of green fluorescent protein, 50°C, assay at pH 7.0 | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | folding of malate dehydrogenase, 50°C, assay at pH 7.0 | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
CTP + H2O + a folded polypeptide | 5 mM Mg2+, 90 min, 0.1 mM released phosphate | Thermoplasma acidophilum | CDP + phosphate + an unfolded polypeptide | - |
? | |
GTP + H2O + a folded polypeptide | 5 mM Mg2+, 0.07 mM released phosphate after 90 min | Thermoplasma acidophilum | GDP + phosphate + an unfolded polypeptide | - |
? | |
additional information | additional ATP-dependent protein refolding activity and suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum | ? | - |
? | |
UTP + H2O + a folded polypeptide | 5 mM Mg2+, 90 min, 0.11 mM released phosphate | Thermoplasma acidophilum | UDP + phosphate + an unfolded polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
archael group II chaperonin | - |
Thermoplasma acidophilum |
chaperonin alpha subunit | - |
Thermoplasma acidophilum |
Ta-cpn alpha | - |
Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
refolding of malate dehydrogenase or green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum |
60 | - |
nucleotide hydrolysis activity | Thermoplasma acidophilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 0.15 mM released phosphate at 40°C, 0.35 mM released phosphate at 70°C | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermoplasma acidophilum |