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Literature summary for 5.6.1.7 extracted from
- Glu257 in GroEL is a sensor involved in coupling polypeptide substrate binding to stimulation of ATP hydrolysis (2006), Protein Sci., 15, 1270-1276.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | nonfolded protein substrate binding-induced stimulation of ATPase activity | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F44W | wild-type variant in which the F44W mutation is introduced so that ATP-induced conformation changes can be followed by monitoring time-resolved changes in fluorescence | Escherichia coli |
| F44W/E257A | the mutation E257A abolishes the nonfolded protein substrate binding-induced stimulation of ATPase activity | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57500 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | oligomer of 14 identical subunits of 57.5 kDa that form two stacked back-to-back heptameric rings | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GroEl | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
ATPase assay | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ATPase assay | Escherichia coli |
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Release 2023.1 (January 2023)
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