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Literature summary for 5.6.1.7 extracted from
- Significance of the N-terminal domain for the function of chloroplast cpn20 chaperonin (2007), J. Biol. Chem., 282, 4463-4469.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GroEL in pTRC99a is overexpressed in Escherichia coli DH5alpha cells | Escherichia coli |
| the N-cpn20 and C-cpn20 homologs of mature wild type cpn20 are cloned individually into the pGEM T-easy vector, the constructs are further cloned into pET22b+ | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
monomer | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using two ion-exchange dimensions followed by gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
GroEL ATPase activity is determined in the presence of the co-chaperonin GroES and the Arabidopsis thaliana co-chaperonin cpn20 and its mutants G32A, G130A, L35A, L133A, G24A and L27A | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | GroEL is a cylindrical oligomer constructed of two back-to-back heptameric rings of 60 kDa subunits each | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GroEl | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
ATPase hydrolysis assay | Escherichia coli |
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Release 2023.1 (January 2023)
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