Literature summary for 5.6.1.7 extracted from

Yoshida, T.; Iizuka, R.; Itami, K.; Yasunaga, T.; Sakuraba, H.; Ohshima, T.; Yohda, M.; Maruyama, T.
Comparative analysis of the protein folding activities of two chaperonin subunits of Thermococcus strain KS-1: the effects of beryllium fluoride (2007), Extremophiles, 11, 225-235.

Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
the chaperonin subunit is expressed in Escherichia coli strain BL21DE3 cells using the expression plasmid pK1Ebeta	Thermococcus sp.
the chaperonin subunits are expressed in Escherichia coli strain BL21DE3 cells using the expression plasmid pK1Ealpha and pK1Ealpha2 for the alpha-subunit mutant and the wild-type alpha-subunit	Thermococcus sp.

Protein Variants

Protein Variants	Comment	Organism
trapalpha	mutant, traps the unfolded and partially folded substrate protein	Thermococcus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
beryllium fluoride	BeFx	Thermococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a folded polypeptide	Thermococcus sp.	-	ADP + phosphate + an unfolded polypeptide	-	ir

Organism

Organism	UniProt	Comment	Textmining
Thermococcus sp.	-	Thermococcus strain KS-1	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a folded polypeptide	-	Thermococcus sp.	ADP + phosphate + an unfolded polypeptide	-	ir

Subunits

Subunits	Comment	Organism
homooligomer	alpha16mer	Thermococcus sp.
homooligomer	beta16mer	Thermococcus sp.

Synonyms

Synonyms	Comment	Organism
chaperonin alpha subunit	-	Thermococcus sp.
chaperonin beta subunit	-	Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	ATPase assay	Thermococcus sp.
90	-	higher than 90°C	Thermococcus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	ATPase assay	Thermococcus sp.

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Release 2023.1 (January 2023)