Literature summary for 5.6.1.7 extracted from

Tang, Y.C.; Chang, H.C.; Chakraborty, K.; Hartl, F.U.; Hayer-Hartl, M.
Essential role of the chaperonin folding compartment in vivo (2008), EMBO J., 27, 1458-1468.

Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
GroEL mutants are constructed in a pCH vector backbone, chaperonin constructs for in vivo co-expression are prepared by inserting the fragments into the pOFXtac-SL2 vector, furthermore the vectors pCH-L16-GFP and pBAD18 are used	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D398A	ATPase deficient mutant	Escherichia coli
EL-2GGM4	cavity size mutant, cis-cavity volume 96%, net charge -42	Escherichia coli
EL-2GGM4-D398A	ATPase deficient mutant	Escherichia coli
EL-3GGM4	cavity size mutant, cis-cavity volume 91%, net charge -42	Escherichia coli
EL-3GGM4-D398A	ATPase deficient mutant	Escherichia coli
EL-3N3Q	charge mutant, cis-cavity volume 100%, net charge 0	Escherichia coli
EL-4GGM4	cavity size mutant, cis-cavity volume 87%, net charge -42	Escherichia coli
EL-KKK2	charge mutant, cis-cavity volume 100%, net charge 0	Escherichia coli
EL-NNQ	charge mutant, cis-cavity volume 100%, net charge -21	Escherichia coli
ELDELTAC	cavity size mutant, cis-cavity volume 104%, net charge -42	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Escherichia coli	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	monomer, GroEL is an 800 kDa cylindrical complex consisting of two stacked heptameric rings of 57 kDa subunits	Escherichia coli
800000	-	GroEL, two stacked heptameric rings of 57 kDa subunits	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a folded polypeptide	Escherichia coli	-	ADP + phosphate + an unfolded polypeptide	-	ir

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a folded polypeptide	-	Escherichia coli	ADP + phosphate + an unfolded polypeptide	-	ir

Subunits

Subunits	Comment	Organism
heptamer	GroEL is an 800 kDa cylindrical complex consisting of two stacked heptameric rings of 57 kDa subunits	Escherichia coli

Synonyms

Synonyms	Comment	Organism
chaperonin ATPase	-	Escherichia coli
GroEL ATPase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	ATPase assay	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	ATPase assay	Escherichia coli

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Release 2023.1 (January 2023)