Literature summary for 5.6.1.7 extracted from

Jung, G.H.; Ahn, T.I.
Purification and characterization of the GroESLx chaperonins from the symbiotic X-bacteria in Amoeba proteus (2001), Protein Expr. Purif., 23, 459-467.

Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression Escherichia coli	Amoeba proteus

Inhibitors

Inhibitors	Comment	Organism	Structure
GroESx	approx. 40% inhibition above a molar ratio of 1 to 1	Amoeba proteus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	14 * 60000, SDS-PAGE	Amoeba proteus
800000	-	sucrose density gradient ultracentrifugation	Amoeba proteus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a folded polypeptide	Amoeba proteus	-	ADP + phosphate + an unfolded polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Amoeba proteus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GroELx	Amoeba proteus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.053	-	at 37°C	Amoeba proteus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a folded polypeptide	-	Amoeba proteus	ADP + phosphate + an unfolded polypeptide	-	?

Subunits

Subunits	Comment	Organism
tetradecamer	14 * 60000, SDS-PAGE	Amoeba proteus

Synonyms

Synonyms	Comment	Organism
GroELx	-	Amoeba proteus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	coagulation above 55°C	Amoeba proteus

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Release 2023.1 (January 2023)