Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | approx. 50% inhibition of CTT complex at 200 mM, approx. 80% inhibition at 800 mM | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | strong activation of CTT complex, almost no activity at 800 mM, maximal activity above 3.2 M | Haloferax volcanii | |
Mg2+ | absolutely required for activity of CTT complex | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76200 | - |
x * 76200, immunoblot | Haloferax volcanii |
86300 | - |
x * 86300, immunoblot | Haloferax volcanii |
95400 | - |
x * 95400, immunoblot | Haloferax volcanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Haloferax volcanii | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | O30560 | - |
- |
Haloferax volcanii | O30561 | - |
- |
Haloferax volcanii | Q9HHA2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Haloferax volcanii | ADP + phosphate + an unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76200, immunoblot | Haloferax volcanii |
? | x * 86300, immunoblot | Haloferax volcanii |
? | x * 95400, immunoblot | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
CCT1 | thermosome subunit 1 | Haloferax volcanii |
CCT2 | thermosome subunit 2, has weak ATPase activity | Haloferax volcanii |
CCT3 | - |
Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Haloferax volcanii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 85 | CTT complex, approx. 50% of maximal activity at 70°C, approx. 80% of maximal activity at 75°C, in the presence of 2.9 N KCl | Haloferax volcanii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
no loss of activity after 30 min | Haloferax volcanii |