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Literature summary for 5.6.1.7 extracted from
- Three GroEL homologues from Rhizobium leguminosarum have distinct in vitro properties (2004), Biochem. Biophys. Res. Commun., 324, 822-828.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
| expression in Escherichia coli | Rhizobium leguminosarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
? |  |
| ATP + H2O + a folded polypeptide | Rhizobium leguminosarum | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Rhizobium leguminosarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Cpn60-1 | Rhizobium leguminosarum |
| recombinant Cpn60-2 | Rhizobium leguminosarum |
| recombinant Cpn60-3 | Rhizobium leguminosarum |
| recombinant GroEL | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
? | |
| ATP + H2O + a folded polypeptide | - |
Rhizobium leguminosarum | ADP + phosphate + an unfolded polypeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cpn60-1 | - |
Rhizobium leguminosarum |
| Cpn60-2 | - |
Rhizobium leguminosarum |
| Cpn60-3 | - |
Rhizobium leguminosarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Escherichia coli |
| 60 | - |
- |
Rhizobium leguminosarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | approx. 15% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C | Escherichia coli |
| 25 | 70 | approx. 20% of maximal activity at 40°C and 70°C, respectively | Rhizobium leguminosarum |
| 25 | 70 | approx. 20% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C | Rhizobium leguminosarum |
| 25 | 70 | approx. 60% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C | Rhizobium leguminosarum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
ATP | 20°C | Rhizobium leguminosarum | |
| 0.009 | - |
ATP | 20°C | Rhizobium leguminosarum | |
| 0.014 | - |
ATP | 20°C | Rhizobium leguminosarum | |
| 0.025 | - |
ATP | 20°C | Escherichia coli | |
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Release 2023.1 (January 2023)
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