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Literature summary for 5.6.1.6 extracted from

  • Chen, J.H.; Xu, W.; Sheppard, D.N.
    Altering intracellular pH reveals the kinetic basis of intraburst gating in the CFTR Cl- channel (2017), J. Physiol., 595, 1059-1076 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D572N the mutation disrupts the interaction of ATP with ATP-binding site 1 Homo sapiens
G1349D the mutation is associated with cystic fibrosis and greatly disturbs enzyme intraburst gating Homo sapiens
K464A the mutation disrupts the interaction of ATP with ATP-binding site 1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + closed Cl- channel Homo sapiens
-
 ADP + phosphate + open Cl- channel
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + closed Cl- channel
-
 Homo sapiens ADP + phosphate + open Cl- channel
-
 ?

Synonyms

Synonyms Comment Organism
CFTR
-
 Homo sapiens
cystic fibrosis transmembrane conductance regulator
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 8.8 when compared with the control (pH 7.3), acidifying pH to 6.3 or alkalinizing pH to 8.3 and 8.8 causes small reductions in the open-time constant of wild type enzyme. By contrast, the fast closed-time constant which describes the short-lived closures that interrupt open bursts, is greatly increased at pH 5.8 and 6.3 Homo sapiens