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Literature summary for 5.6.1.6 extracted from

  • Zhang, J.; Hwang, T.C.
    The fifth transmembrane segment of cystic fibrosis transmembrane conductance regulator contributes to its anion permeation pathway (2015), Biochemistry, 54, 3839-3850.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.6

Protein Variants

Protein Variants Comment Organism
A299C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens
A326C site-directed mutagenesis, the substituted cysteine does not respond to neither internal nor external 2-sulfonatoethyl methanethiosulfonate and is inaccessible to channel-permeant thiol-specific reagent [Au(CN)2]- Homo sapiens
F310C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens
F311C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens
L323C site-directed mutagenesis, the substituted cysteine does not respond to neither internal nor external 2-sulfonatoethyl methanethiosulfonate and is inaccessible to channel-permeant thiol-specific reagent [Au(CN)2]- Homo sapiens
N306C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens
R303C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens
S307C site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-sulfonatoethyl methanethiosulfonate enzyme residues A299, R303, N306, S307, F310, and F311 are accessible to intracellular 2-sulfonatoethyl methanethiosulfonate in both the open and closed states Homo sapiens
glibencalmide an open-channel blocker Homo sapiens
[Au(CN)2]- a channel-permeant thiol-specific reagent Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme possesses two transmembrane domains, each of which comprises six transmembrane segments. Function of several transmembrane segments (TMs), including TM1, TM3, TM6, TM9, TM11, and TM12, as pore-lining segments the enzyme. Screening of the entire transmembrane segment 5 defined by the original topology model and its cytoplasmic extension in a Cys-less background, the segment of TM5 between F311 and L323 is concealed from the pore by other TMs and/or lipid bilayers, the cytoplasmic portion of TM5 lines the pore Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P13569
-
-

Synonyms

Synonyms Comment Organism
CFTR
-
 Homo sapiens
cystic fibrosis transmembrane conductance regulator
-
 Homo sapiens

General Information

General Information Comment Organism
additional information enzyme residues A299, R303, N306, S307, F310, and F311 are accessible to intracellular 2-sulfonatoethyl methanethiosulfonate in both the open and closed states. The segment of transmembrane segment 5 between F311 and L323 is concealed from the pore by other TMs and/or lipid bilayers, the cytoplasmic portion of TM5 lines the pore Homo sapiens