Literature summary for 5.6.1.6 extracted from

Rosenberg, M.F.; Kamis, A.B.; Aleksandrov, L.A.; Ford, R.C.; Riordan, J.R.
Purification and crystallization of the cystic fibrosis transmembrane conductance regulator (CFTR) (2004), J. Biol. Chem., 279, 39051-39057.

Cloned(Commentary)

Cloned (Comment)	Organism
C terminally deca-histidine-tagged wild type human CFTR epxressed in baby hamster kidney-21 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
by hanging drop method, at 20 A resolution in the xy plane, at 30 A resolution along z plane, two crystal forms, one with a roughly hexagonal profile, the other with an opened-out triangular profile, the two conformations are in presence of a nucleotide, which may be related to the role of CFTR as an ion channel rather than a transporter, and may represent the open and closed states of the channel	Homo sapiens

Crystallization (Comment)

Organism

by hanging drop method, at 20 A resolution in the xy plane, at 30 A resolution along z plane, two crystal forms, one with a roughly hexagonal profile, the other with an opened-out triangular profile, the two conformations are in presence of a nucleotide, which may be related to the role of CFTR as an ion channel rather than a transporter, and may represent the open and closed states of the channel

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA matrix and subsequent WGA-agorse affinity step	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.06	-	with ATP as substrate	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + closed Cl- channel	-	Homo sapiens	ADP + phosphate + open Cl- channel	-	?
GTP + H2O + closed Cl- channel	-	Homo sapiens	GDP + phosphate + open Cl- channel	-	?

Synonyms

Synonyms	Comment	Organism
CFTR	-	Homo sapiens
cystic fibrosis transmembrane conductance regulator	-	Homo sapiens