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Literature summary for 5.6.1.5 extracted from

  • Guzman-Rodriguez, M.; de la Rosa, A.P.; Santos, L.
    Characterization of ATPase activity of the AAA ARC from Bifidobacterium longum subsp. infantis (2015), Acta Biochim. Pol., 62, 221-227 .
    View publication on PubMed
Search for more literature for 5.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene arc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant overexpression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21pLysS Bifidobacterium longum subsp. infantis

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Bifidobacterium longum subsp. infantis
alpha,beta-methyleneadenosine 5'-triphosphate
-
 Bifidobacterium longum subsp. infantis
EDTA
-
 Bifidobacterium longum subsp. infantis
N-ethylmaleimide
-
 Bifidobacterium longum subsp. infantis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.327
-
 ATP pH 5.0, 37°C, recombinant enzyme Bifidobacterium longum subsp. infantis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 3% activity compared to Mg2+ Bifidobacterium longum subsp. infantis
Co2+ 9% activity compared to Mg2+ Bifidobacterium longum subsp. infantis
Cu2+ 12% activity compared to Mg2+ Bifidobacterium longum subsp. infantis
Mg2+ dependent on, required for catalytic activity Bifidobacterium longum subsp. infantis
Mn2+ 34% activity compared to Mg2+ Bifidobacterium longum subsp. infantis
Zn2+ 62% activity compared to Mg2+ Bifidobacterium longum subsp. infantis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Bifidobacterium longum subsp. infantis
-
 ADP + phosphate
-
 ?
ATP + H2O Bifidobacterium longum subsp. infantis S12
-
 ADP + phosphate
-
 ?
ATP + H2O Bifidobacterium longum subsp. infantis JCM 1222
-
 ADP + phosphate
-
 ?
ATP + H2O Bifidobacterium longum subsp. infantis NCTC 11817
-
 ADP + phosphate
-
 ?
ATP + H2O Bifidobacterium longum subsp. infantis DSM 20088
-
 ADP + phosphate
-
 ?
ATP + H2O Bifidobacterium longum subsp. infantis ATCC 15697
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. infantis B7GUP3
-
-
Bifidobacterium longum subsp. infantis ATCC 15697 B7GUP3
-
-
Bifidobacterium longum subsp. infantis DSM 20088 B7GUP3
-
-
Bifidobacterium longum subsp. infantis JCM 1222 B7GUP3
-
-
Bifidobacterium longum subsp. infantis NCTC 11817 B7GUP3
-
-
Bifidobacterium longum subsp. infantis S12 B7GUP3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Bifidobacterium longum subsp. infantis ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis ADP + phosphate
-
 ?
ATP + H2O
-
 Bifidobacterium longum subsp. infantis S12 ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis S12 ADP + phosphate
-
 ?
ATP + H2O
-
 Bifidobacterium longum subsp. infantis JCM 1222 ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis JCM 1222 ADP + phosphate
-
 ?
ATP + H2O
-
 Bifidobacterium longum subsp. infantis NCTC 11817 ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis NCTC 11817 ADP + phosphate
-
 ?
ATP + H2O
-
 Bifidobacterium longum subsp. infantis DSM 20088 ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis DSM 20088 ADP + phosphate
-
 ?
ATP + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ADP + phosphate
-
 ?
ATP + H2O highly preferred substrate Bifidobacterium longum subsp. infantis ATCC 15697 ADP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis CDP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis S12 CDP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis JCM 1222 CDP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis NCTC 11817 CDP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis DSM 20088 CDP + phosphate
-
 ?
CTP + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 CDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis GDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis S12 GDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis JCM 1222 GDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis NCTC 11817 GDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis DSM 20088 GDP + phosphate
-
 ?
GTP + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 GDP + phosphate
-
 ?
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis ?
-
-
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis S12 ?
-
-
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis JCM 1222 ?
-
-
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis NCTC 11817 ?
-
-
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis DSM 20088 ?
-
-
additional information Bl-ARC has a preference for ATP as substrate (100%) when compared against GTP (28%), CTP (31%), and UTP (1.5%) Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
-

Subunits

Subunits Comment Organism
homohexamer 6 * 56000, recombinant His6-tagged enzyme, SDS-PAGE Bifidobacterium longum subsp. infantis

Synonyms

Synonyms Comment Organism
AAA ARC
-
 Bifidobacterium longum subsp. infantis
AAA ATPase forming ring-shaped complex UniProt Bifidobacterium longum subsp. infantis
ARC
-
 Bifidobacterium longum subsp. infantis
ATPase
-
 Bifidobacterium longum subsp. infantis
Bl-ARC
-
 Bifidobacterium longum subsp. infantis
proteasomal ATPase
-
 Bifidobacterium longum subsp. infantis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bifidobacterium longum subsp. infantis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 activity range Bifidobacterium longum subsp. infantis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bifidobacterium longum subsp. infantis

General Information

General Information Comment Organism
evolution the enzyme contains an AAA ATPase domain, phylogenetic analysis Bifidobacterium longum subsp. infantis
additional information overall 3D structure prediction of the AAA Bl-ARC obtained by homology modeling using the Mycobacterium tuberculosis ARC crystal interdomain structure Bifidobacterium longum subsp. infantis
physiological function possible function of enzyme Bl-ARC as a chaperone in the degradation pathway via pupylation. The enzyme shows a possible AAA unfoldase activity for target substrates prior to degradation Bifidobacterium longum subsp. infantis