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Literature summary for 5.6.1.5 extracted from

  • Wu, Y.; Hu, K.; Li, D.; Bai, L.; Yang, S.; Jastrab, J.B.; Xiao, S.; Hu, Y.; Zhang, S.; Darwin, K.H.; Wang, T.; Li, H.
    Mycobacterium tuberculosis proteasomal ATPase Mpa has a beta-grasp domain that hinders docking with the proteasome core protease (2017), Mol. Microbiol., 105, 227-241 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and ER2566 cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
apo and ADP-bound enzyme forms, sitting or hanging drop vapor diffusion methods, using 0.1 M Tris-HCl, pH 8.2, 20% (w/v) PEG 400, 0.2M MgCl2 or 100 mM Bis-Tris propane, pH 7.0, 0.475 M NaCl, 18.2% (w/v) PEG 4000 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20 mM used in assay conditions Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + polypeptide Myc-Pup-FabD-his
-
 Mycobacterium tuberculosis ADP + phosphate + unfolded polypeptide Myc-Pup-FabD-his
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
mpA
-
 Mycobacterium tuberculosis
proteasomal adenosine triphosphatase
-
 Mycobacterium tuberculosis
proteasomal ATPase
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism the enzyme captures, unfolds, and translocates protein substrates into the 20S proteasome core particle for degradation Mycobacterium tuberculosis