Protein Variants | Comment | Organism |
---|---|---|
G360A/G387A | site-directed mutagenesis, the Rpt6 mutant reduces the Rpn14 association with proteasome base and disrupts holoenzyme formation in vitro | Saccharomyces cerevisiae |
additional information | Saccharomyces cerevisiae Rpt6-C spans P318-K405 and is a protein with one conformational state displays in the resulting spectrum one backbone amide signal for each amino acid except proline. Generation Rpt6-C protein samples in which lysine, alanine, leucine, or valine were selectively 15N-labeled. Rpt6-C exchanges between a 4-helix bundle and a partially unfolded state that exhibits increased dynamics, Rpt6-C reversibly exchanges between its two conformational states | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + polypeptide | Saccharomyces cerevisiae | - |
ADP + phosphate + unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q01939 | gene RPT6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + polypeptide | - |
Saccharomyces cerevisiae | ADP + phosphate + unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three Rpt heterodimeric RP assembly intermediates occur along with four chaperone-like proteins that interact transiently with C-terminal domains of Rpt proteins | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
RP triphosphatase protein | - |
Saccharomyces cerevisiae |
Rpt6 | - |
Saccharomyces cerevisiae |
Rpt6 ATPase | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of Rpt6's partially unfolded state by glycine substitution in Rpt6 mutant G360A/G387A disrupts holoenzyme formation in vitro, an effect enhanced by Rpn14. Loss of Rpt6 conformational exchange enhances Rpn14 requirement during proteasome stress. Saccharomyces cerevisiae lacking Rpn14 and with Rpt6 mutant G360A/G387A incorporated demonstrate hallmarks of defective proteasome assembly and synthetic growth defects | Saccharomyces cerevisiae |
additional information | Rpt6's C-terminal domain undergoes dynamic helix-coil transitions enabled by helix-destabilizing glycines within its two most C-terminal alpha-helices. Rpn14 binds selectively to Rpt6's 4-helix bundle, with surprisingly high affinity. The N-terminal ends of alpha2 and alpha4, and the alpha1-alpha2 and alpha3-alpha4 loops are involved in Rpn14-binding | Saccharomyces cerevisiae |
physiological function | juxtaposed to either or both ends of the proteasome core particle a 19S regulatory particle (RP) exists that recognizes and prepares ubiquitinated proteins for proteolysis. RP triphosphatase proteins (Rpt1-Rpt6), which are critical for substrate translocation into the CP, bind chaperone-like proteins (Hsm3, Nas2, Nas6, Rpn14) implicated in RP assembly, effect of Rpn14 and its interaction with Rpt6 during assembly, overview | Saccharomyces cerevisiae |