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Literature summary for 5.6.1.5 extracted from

  • Reuter, C.J.; Kaczowka, S.J.; Maupin-Furlow, J.A.
    Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii (2004), J. Bacteriol., 186, 7763-7772.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene panA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Haloferax volcanii
gene panB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45410
-
x * 45410, amino acid sequence calculation Haloferax volcanii
45664
-
x * 45664, amino acid sequence calculation Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GUJ7 PanA; isozyme PanA
-
Haloferax volcanii Q5UT56 PanB; isozyme PanB
-
Haloferax volcanii DS2 D4GUJ7 PanA; isozyme PanA
-
Haloferax volcanii DS2 Q5UT56 PanB; isozyme PanB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Haloferax volcanii

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression level during growth phases Haloferax volcanii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the Pan proteins are part of the 5-member proteasome isoforms, the five proteins are differentially regulated by a posttranscriptional mechanism, e.g. PanA remains relatively constant, a coiled-coil structure at the N-terminus of Pan is involved in substrate recognition Haloferax volcanii ?
-
?
additional information the Pan proteins are part of the 5-member proteasome isoforms, the five proteins are differentially regulated by a posttranscriptional mechanism, e.g. PanB increases 7fold in the stationary phase, a coiled-coil structure at the N-terminus of Pan is involved in substrate recognition Haloferax volcanii ?
-
?
additional information the Pan proteins are part of the 5-member proteasome isoforms, the five proteins are differentially regulated by a posttranscriptional mechanism, e.g. PanA remains relatively constant, a coiled-coil structure at the N-terminus of Pan is involved in substrate recognition Haloferax volcanii DS2 ?
-
?
additional information the Pan proteins are part of the 5-member proteasome isoforms, the five proteins are differentially regulated by a posttranscriptional mechanism, e.g. PanB increases 7fold in the stationary phase, a coiled-coil structure at the N-terminus of Pan is involved in substrate recognition Haloferax volcanii DS2 ?
-
?

Subunits

Subunits Comment Organism
? x * 45410, amino acid sequence calculation Haloferax volcanii
? x * 45664, amino acid sequence calculation Haloferax volcanii
More the Pan proteins are part of the 5-member proteasome isoforms, the five proteins are differentially regulated, a coiled-coil structure at the N-terminus of Pan is involved in substrate recognition Haloferax volcanii

Synonyms

Synonyms Comment Organism
PanA
-
Haloferax volcanii
PanB
-
Haloferax volcanii
proteasome-activating nucleotidase
-
Haloferax volcanii

pI Value

Organism Comment pI Value Maximum pI Value
Haloferax volcanii sequence calculation
-
4.3
Haloferax volcanii sequence calculation
-
4.5