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Literature summary for 5.6.1.5 extracted from
- Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome (2000), J. Bacteriol., 182, 1680-1692.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Methanocaldococcus jannaschii |  |
| N-ethylmaleimide | - |
Methanocaldococcus jannaschii | |
| p-chloromercuriphenyl-sulfonic acid | - |
Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
ATP | HslU | Escherichia coli | |
| 0.307 | - |
CTP | PAN | Methanocaldococcus jannaschii | |
| 0.477 | - |
ATP | PAN(DELTA1-73) | Methanocaldococcus jannaschii | |
| 0.493 | - |
CTP | PAN(DELTA1-73) | Methanocaldococcus jannaschii | |
| 0.497 | - |
ATP | PAN | Methanocaldococcus jannaschii | |
| 0.55 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 650000 | - |
PAN, proteasome-activating nucleotidase, complex with ATPase activity, gel filtration | Methanocaldococcus jannaschii |
| 700000 | - |
- |
Saccharomyces cerevisiae |
| 700000 | - |
- |
Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + polypeptide | Escherichia coli | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Saccharomyces cerevisiae | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Trypanosoma brucei | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Rhodococcus erythropolis | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Trichormus variabilis | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Methanocaldococcus jannaschii | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | Methanosarcina thermophila | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanosarcina thermophila | - |
- |
- |
| Rhodococcus erythropolis | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Trichormus variabilis | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 700-kDa complex | Methanocaldococcus jannaschii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Escherichia coli | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Saccharomyces cerevisiae | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Trypanosoma brucei | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Rhodococcus erythropolis | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Trichormus variabilis | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanocaldococcus jannaschii | |
| ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + polypeptide | - |
Escherichia coli | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Saccharomyces cerevisiae | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Trypanosoma brucei | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Rhodococcus erythropolis | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Trichormus variabilis | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | ADP + phosphate + unfolded polypeptide | - |
? | |
| ATP + H2O + polypeptide | - |
Methanosarcina thermophila | ADP + phosphate + unfolded polypeptide | - |
? | |
| CTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | CDP + phosphate + unfolded polypeptide | - |
? | |
| GTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | GDP + phosphate + unfolded polypeptide | - |
? | |
| ITP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | IDP + phosphate + unfolded polypeptide | - |
? | |
| TTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | TDP + phosphate + unfolded polypeptide | - |
? | |
| UTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | UDP + phosphate + unfolded polypeptide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
PAN(DELTA1-73) | Methanocaldococcus jannaschii |
| 80 | - |
full-length protein | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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