Literature summary for 5.6.1.4 extracted from

Dietrich, D.; Seiler, F.; Essmann, F.; Dodt, G.
Identification of the kinesin KifC3 as a new player for positioning of peroxisomes and other organelles in mammalian cells (2013), Biochim. Biophys. Acta, 1833, 3013-3024.

Search for more literature for 5.6.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
the gene encoding the enzyme is located on chromosome 16q13-q21, transient overexpression of enzyme KifC3 in COS-7 cells, KifC3 is localized near the nucleus and concentrated at perinuclear microtubule organizing center, overexpression of KifC3 did not affect peroxisomal distribution. Coexpression with human Myc-tagged PEX1	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme knockdown using KifC3-mRNA targeting siRNA causing reduced KifC3 function in peroxisomal motility, the peroxisomal KifC3 knockdown phenotype depends on microtubules, is independent of the cell phase and also affects the morphology of mitochondria and endoplasmic reticulum	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microtubule	-	Homo sapiens	5874	-
additional information	endogenous KifC3 as well as overexpressed KifC3 cannot be detected at peroxisomes. Overall peroxisomal distribution is not affected by overexpression of either human KifC3	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	Homo sapiens	-	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?
additional information	Homo sapiens	interaction of KifC3 and the AAA-protein PEX1, an ATPase associated with various cellular activities	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
A-549 cell	-	Homo sapiens	-
fibroblast	-	Homo sapiens	-
additional information	ubiquitous expression	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Homo sapiens	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?
additional information	interaction of KifC3 and the AAA-protein PEX1, an ATPase associated with various cellular activities	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
C-terminal kinesin	-	Homo sapiens
KIFC3	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	enzyme KifC3 is a C-terminal kinesin of the kinesin-14 family	Homo sapiens
malfunction	knockdown of KifC3 using RNAi results in an increase of cells with perinuclear-clustered peroxisomes, indicating enhanced minus-end directed motility of peroxisomes leading to peroxisomal clustering at the microtubule-organizing center. The peroxisomal phenotype is cell cycle phase independent, while microtubules are essential for phenotype formation	Homo sapiens
metabolism	compared to dynein, representing a minus-motor and therefore transporting cargoes to the cell center, the kinesin's direction of transport is dependent on the position of their motor domain. C-kinesins are typically minus-motors. Transport of peroxisomes along microtubules in mammalian cells occurs by the motor proteins cytoplasmic dynein and kinesin-1 The enzyme KifC3 knockdown also affects the morphology of mitochondria and endoplasmic reticulum, phenotype	Homo sapiens
physiological function	KifC3 is able to bind to microtubules and most likely functions as a microtubule motor protein. Enzyme KifC3 may play a regulatory role in minus-end directed peroxisomal transport for example by blocking the motor function of dynein at peroxisomes	Homo sapiens

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Release 2023.1 (January 2023)