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Literature summary for 5.6.1.4 extracted from

  • Braun, M.; Drummond, D.R.; Cross, R.A.; McAinsh, A.D.
    The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism (2009), Nat. Cell Biol., 11, 724-730.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
microtubule the solution Klp2 ATPase is activated 15fold more effectively by Schizosaccharomyces pombe microtubules than by pig brain microtubules Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
182000
-
theoretical molecular weight Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe in microtubule gliding assays using microtubules assembled from pig brain tubulin, Klp2 drives minus-end-directed motility ?
-
?
additional information Schizosaccharomyces pombe Klp2 is required for the proper organization of interphase microtubules into bipolar arrays of parallel-overlapped microtubules ?
-
?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
6His-tagged full-length Klp2. a Klp2 protein lacking the motor domain (Klp2-tail) and a Klp2 protein containing only the motor domain and a short coiled coil to mediate dimerization (Klp2-motor) are purified by ammonium sulfate precipitation and HisTrapHP column chromatography Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
Schizosaccharomyces pombe ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
additional information in microtubule gliding assays using microtubules assembled from pig brain tubulin, Klp2 drives minus-end-directed motility Schizosaccharomyces pombe ?
-
?
additional information Klp2 is required for the proper organization of interphase microtubules into bipolar arrays of parallel-overlapped microtubules Schizosaccharomyces pombe ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
kinesin-14
-
Schizosaccharomyces pombe
Klp2
-
Schizosaccharomyces pombe

pI Value

Organism Comment pI Value Maximum pI Value
Schizosaccharomyces pombe theoretical pI-value
-
10