Activating Compound | Comment | Organism | Structure |
---|---|---|---|
microtubule | in presence of microtubule, ncd ATPase activity is stimulated by ca. 30fold | Drosophila sp. (in: flies) | |
microtubules | stimulates ncd ATPase activity by ca. 30fold | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
PCR from a ncd cDNA, ncd cloned, polypeptide expressed in Escherichia coli | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in contrast to cytoplasmic dynein, ncd polypeptide is not cleaved by UV-vanadate treatment, and its ATPase and motility are unaffected by vanadate (up to 0.1 mM) | Drosophila sp. (in: flies) | |
V5+ | dynein | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
microtubule | - |
Drosophila sp. (in: flies) | 5874 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | maximal stimulation at 0.15-0.2 M | Drosophila sp. (in: flies) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
ncd polypeptide, SDS-PAGE | Drosophila melanogaster |
80000 | - |
ncd | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | Drosophila melanogaster | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | Bos taurus | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | Doryteuthis pealeii | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | Tetrahymena sp. | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Doryteuthis pealeii | - |
squid | - |
Drosophila melanogaster | - |
- |
- |
Drosophila sp. (in: flies) | - |
recombinantly expressed in Escherichia coli | - |
Tetrahymena sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, recombinant protein, expressed in Escherichia coli | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Drosophila melanogaster | |
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Bos taurus | |
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Doryteuthis pealeii | |
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Tetrahymena sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-80°C, 0.3 M NaCl, stored in liquid nitrogen | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxy-ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | 2',3'-dideoxy-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
2'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
2'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
2'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Doryteuthis pealeii | 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
3'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
3'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
3'-dATP + H2O + a kinesin associated with a microtubule at position n | - |
Doryteuthis pealeii | 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Bos taurus | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Doryteuthis pealeii | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Tetrahymena sp. | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
additional information | GTP is no substrate | Drosophila melanogaster | ? | - |
? | |
additional information | GTP is no substrate | Doryteuthis pealeii | ? | - |
? | |
N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n | - |
Doryteuthis pealeii | N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | ncd polypeptide expressed in Escherichia coli | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
ncd | - |
Drosophila melanogaster |
ncd | - |
Drosophila sp. (in: flies) |
ncd | - |
Doryteuthis pealeii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Drosophila sp. (in: flies) |
7 | - |
ncd and cytoplasmic dynein | Drosophila melanogaster |