Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6 tagged enzyme mutant W127C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W127C | site-directed mutagenesis, cysteine-light Eg5 monomer, the cysteine is located in the center of L5 and replaces a tryptophan residue that makes a hydrophobic ring stacking interaction with Tyr211 in alpha3 in AMPPNP, the mutant shows slowlier product release compared to the wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP-PNP | binding structure | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state and transient kinetics studies of fluorescence-labeled W127C mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | Homo sapiens | - |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Eg5 | - |
Homo sapiens |
mitotic kinesin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a mmember of the kinesin superfamily of molecular motors | Homo sapiens |
additional information | loop L5, the longest in the mitotic kinesin Eg5, assumes three distinct orientations during the ATPase cycle of the mitotic kinesin Eg5, L5 is in a dynamic equilibrium of three conformations whose relative proportions shift during the ATPase cycle. Loop L5 shapes the enzymology of kinesin motors to meet specific physiologic roles | Homo sapiens |