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Literature summary for 5.6.1.3 extracted from

  • Muretta, J.M.; Behnke-Parks, W.M.; Major, J.; Petersen, K.J.; Goulet, A.; Moores, C.A.; Thomas, D.D.; Rosenfeld, S.S.
    Loop L5 assumes three distinct orientations during the ATPase cycle of the mitotic kinesin Eg5: a transient and time-resolved fluorescence study (2013), J. Biol. Chem., 288, 34839-34849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His6 tagged enzyme mutant W127C Homo sapiens

Protein Variants

Protein Variants Comment Organism
W127C site-directed mutagenesis, cysteine-light Eg5 monomer, the cysteine is located in the center of L5 and replaces a tryptophan residue that makes a hydrophobic ring stacking interaction with Tyr211 in alpha3 in AMPPNP, the mutant shows slowlier product release compared to the wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP-PNP binding structure Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady state and transient kinetics studies of fluorescence-labeled W127C mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n Homo sapiens
-
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
?

Synonyms

Synonyms Comment Organism
Eg5
-
Homo sapiens
mitotic kinesin
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is a mmember of the kinesin superfamily of molecular motors Homo sapiens
additional information loop L5, the longest in the mitotic kinesin Eg5, assumes three distinct orientations during the ATPase cycle of the mitotic kinesin Eg5, L5 is in a dynamic equilibrium of three conformations whose relative proportions shift during the ATPase cycle. Loop L5 shapes the enzymology of kinesin motors to meet specific physiologic roles Homo sapiens