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Literature summary for 5.6.1.3 extracted from

  • Friel, C.T.; Howard, J.
    The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization (2011), EMBO J., 30, 3928-3939.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
His tagged protein expressed in Sf9 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 methylanthraniloyl-ATP pH 6.9, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cation exchange chromatography, Ni-affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n unconventional ATPase cycle, adapted for microtubule depolymerization, low basal activity is accelerated by tubulin and microtubles Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
methylanthraniloyl-ATP + H2O + a kinesin associated with a microtubule at position n unconventional ATPase cycle, adapted for microtubule depolymerization, low basal activity is accelerated by tubulin and microtubles Homo sapiens methylanthraniloyl-ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Synonyms

Synonyms Comment Organism
kinesin-13 MCAK kinesin-13 subfamily members do not move directionally along microtubules but, instead, depolymerize them Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0009
-
 methylanthraniloyl-ATP basal activity, in absence of tubulin or microtubles, pH 6.9, 25°C Homo sapiens
0.00095
-
 ATP basal activity, in absence of tubulin or microtubles, pH 6.9, 25°C Homo sapiens
0.123
-
 ATP in presence of unpolymerized tubulin, pH 6.9, 25°C Homo sapiens
4.97
-
 ATP in presence of microtubles, pH 6.9, 25°C Homo sapiens