Literature summary for 5.6.1.3 extracted from

Cochran, J.C.; Sontag, C.A.; Maliga, Z.; Kapoor, T.M.; Correia, J.J.; Gilbert, S.P.
Mechanistic analysis of the mitotic kinesin Eg5 (2004), J. Biol. Chem., 279, 38861-38870.

Search for more literature for 5.6.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
microtubules	strong increase in ATPase activity in the presence of microtubules	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminal truncated, His-tagged Eg5-437 and Eg5-367 in Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.019	-	ATP	25°C, pH 7.2, Eg5-437	Homo sapiens
0.025	-	ATP	25°C, pH 7.2, Eg5-367	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42119	-	1 * 42119, deduced from nucleotide sequence, N-terminal truncated, His-tagged Eg5-367	Homo sapiens
44600	-	1 * 44600, sedimentation velocity, N-terminal truncated, His-tagged Eg5-437	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	Homo sapiens	-	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Eg5-437 and Eg5-367	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Homo sapiens	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 42119, deduced from nucleotide sequence, N-terminal truncated, His-tagged Eg5-367	Homo sapiens
monomer	1 * 44600, sedimentation velocity, N-terminal truncated, His-tagged Eg5-437	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Eg5	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.9	-	ATP	25°C, pH 7.2, Eg5-437	Homo sapiens
5.5	-	ATP	25°C, pH 7.2, Eg5-367	Homo sapiens

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Release 2023.1 (January 2023)