Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.3 extracted from

  • Yamazaki, H.; Nakata, T.; Okada, Y.; Hirokawa, N.
    KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport (1995), J. Cell Biol., 130, 1387-1399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
KIF3A and KIF3B, PCR amplified cDNA Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000 85000 KIF3A, gel filtration Mus musculus
85000
-
 recombinant KIF3A, gel filtration Mus musculus
85280
-
 KIF3B, nucleotide sequence analysis Mus musculus
95000
-
 KIF3B, gel filtration Mus musculus
250000
-
 recombinant KIF3A/B complex, gel filtration Mus musculus
350000
-
 native KIF3 complex, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n Mus musculus
-
 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n Bos taurus
-
 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n Chlamydomonas sp.
-
 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n Mus musculus
-
 GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n Bos taurus
-
 GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n Chlamydomonas sp.
-
 GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-
Chlamydomonas sp.
-
-
-
Mus musculus
-
 mouse
-

Purification (Commentary)

Purification (Comment) Organism
KIF3B, recombinant protein expressed in baculovirus-Sf9 expression system Mus musculus

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. Mus musculus
a
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. Bos taurus
a
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. Chlamydomonas sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
brain
-
 Bos taurus
-
embryo
-
 Mus musculus
-
testis
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Mus musculus ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Bos taurus ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Chlamydomonas sp. ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n
-
 Mus musculus GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n
-
 Bos taurus GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n
-
 Chlamydomonas sp. GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Subunits

Subunits Comment Organism
dimer KIF3A + KIF3B Mus musculus

Synonyms

Synonyms Comment Organism
KIF3A
-
 Mus musculus
KIF3B
-
 Mus musculus
murine kinesin
-
 Mus musculus